Abstract: Fe₃O₄/aspergillus Niger were prepared by adding culturing media nanoparticles in Fe₃O₄, then the static adsorption experiment was carried out, Through a static test, different experimental parameters such as solution pH, temperature, dosing, adsorption time and initial concentration were examined to investigate their effects on adsorption of U(Ⅵ) by Fe₃O₄/Aspergillus niger. The results show that pH value can be the important factor for affecting U(Ⅵ). When solution pH=4, 25℃, dosing quantity 1 g/L, U(Ⅵ) of the initial concentration is 10 mg/L, the adsorption rate is as high as 98.89%, adsorption process is verging to be balanced after 15 h. The prepared composites were characterized by SEM, EDS and FTIR. SEM-EDS show that part of the hyphae surface was coated with tiny particles and the structure is the grid. FTIR spectra demonstrate that -OH, -C═O and -CONH interact with U(Ⅵ).