Effect of nano-hydroxyapatite on Mg Zn Zr alloy biological properties in vitro
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摘要: 采用常规熔炼方法在氩气保护和电磁搅拌条件下制备了纳米羟基磷灰石(nHA)/Mg-2.5Zn-0.5Zr(质量分数, %)生物复合材料, 并与基体镁合金对比, 通过与大鼠成骨细胞共培养考察材料的体外生物相容性。结果表明, 2组材料均无明显细胞毒性, 但在倒置相差显微镜下, nHA/Mg-2.5Zn-0.5Zr材料周边细胞形态完好, 密度显著高于基体镁合金组, 前3天培养液中基本无材料溶出物, 5~7天后溶出物少于基体镁合金组。细胞生长曲线显示, 随着培养时间延长2组材料表面的细胞数量均呈上升趋势, 而在复合培养的第3天和第5天, nHA/Mg-2.5Zn-0.5Zr复合材料表面细胞增殖量显著大于基体镁合金组。扫描电镜下可见成骨细胞对2组材料的反应有所不同, 在复合材料表面, 细胞呈梭形或三角形紧密贴伏, 其伪足正常伸展使细胞彼此相连, 7天后完全覆盖了整个试样。相反, 基体镁合金表面细胞呈纺锤状, 显示与材料的细胞吸附性较差。
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关键词:
- 纳米羟基磷灰石 /
- nHA/Mg-2.5Zn-0.5Zr复合材料 /
- 成骨细胞 /
- 体外细胞培养 /
- 细胞增殖
Abstract: The nHA/Mg-2.5Zn-0.5Zr(mass fraction, %)composites, prepared by melting method in an argon gas atmosphere and electromagnetic stirring, were cultured with rat osteoblasts to investigate the biocompatibility in vitro compared to magnesium-matrix material. The results both of the two groups show no obvious cytotoxicity, while the cellular morphology is better and the cell density is higher in the nHA/Mg-2.5Zn-0.5Zr group than in the magnesium-matrix group. The cell growth curve shows osteoblast proliferation is greater on the nHA/Mg-2.5Zn-0.5Zr composites than on the magnesium-matrix material during the prescribed time periods, meanwhile, there are statistical differences between the two groups after 3 and 5 days of culture. Under the SEM observation, the cell responses to the samples were different, the osteoblasts attached very well to the composites with the shape of shuttle and/or triangular, and the cells were in a close contact with each other. The cells in the magnesium-matrix control group appeared with a spindle-shape, which indicated a weak adhesion to the material. -
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