To study the influence of eccentricity on eccentric compressive mechanical performance of glulam bamboo columns, six glulam bamboo columns were tested under eccentric loading. The results show that the failure modes of specimens mainly display as tensile fracture of bamboo fibers in the middle region of columns under eccentric loading. With the increase of eccentricity ratio, the peak load reduces, whereas axial displacement and lateral deflection at the middle cross-section corresponding to the peak load increase, and the flexural deformation is significant. At the peak load point, the bamboo compressive strain of eccentric compression specimens is much greater than that of axial compression specimen, the former is 3.1-4.6 times that of the latter, and the compressive deformation ability and strength are developed fully in the compression zone for glulam bamboo columns under eccentric loading. Based on the testing results and theoretical analysis, the calculation methods to predict the ultimate bearing capacity of the glulam bamboo columns under eccentric compression was proposed, and the mean absolute error is less 5% indicating that the calculated results are consistent with the testing results.