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醛基化透明质酸/羟丙基壳聚糖自愈合水凝胶的制备与性能

王艺璇 曲萌菲 张杭 李征征

王艺璇, 曲萌菲, 张杭, 等. 醛基化透明质酸/羟丙基壳聚糖自愈合水凝胶的制备与性能[J]. 复合材料学报, 2023, 40(10): 5885-5892. doi: 10.13801/j.cnki.fhclxb.20230207.001
引用本文: 王艺璇, 曲萌菲, 张杭, 等. 醛基化透明质酸/羟丙基壳聚糖自愈合水凝胶的制备与性能[J]. 复合材料学报, 2023, 40(10): 5885-5892. doi: 10.13801/j.cnki.fhclxb.20230207.001
WANG Yixuan, QU Mengfei, ZHANG Hang, et al. Preparation and properties of oxidized hyaluronic acid-hydroxypropyl chitosan self-healing hydrogel[J]. Acta Materiae Compositae Sinica, 2023, 40(10): 5885-5892. doi: 10.13801/j.cnki.fhclxb.20230207.001
Citation: WANG Yixuan, QU Mengfei, ZHANG Hang, et al. Preparation and properties of oxidized hyaluronic acid-hydroxypropyl chitosan self-healing hydrogel[J]. Acta Materiae Compositae Sinica, 2023, 40(10): 5885-5892. doi: 10.13801/j.cnki.fhclxb.20230207.001

醛基化透明质酸/羟丙基壳聚糖自愈合水凝胶的制备与性能

doi: 10.13801/j.cnki.fhclxb.20230207.001
基金项目: 天津市海洋资源与化学重点实验室(201706);2018年度天津市教委科研计划项目(2018KJ110);天津市制浆造纸重点实验室开放基金资助项目(201809)
详细信息
    通讯作者:

    李征征,博士,副研究员,硕士生导师,研究方向为智能高分子水凝胶及其生物医用研究 E-mail: li.z.z@tust.edu.cn

  • 中图分类号: O63;TB332

Preparation and properties of oxidized hyaluronic acid-hydroxypropyl chitosan self-healing hydrogel

Funds: Tianjin Key Laboratory of Marine Resources and Chemistry (201706); 2018 Tianjin Education Commission Scientific Research Program Project (2018KJ110); Tianjin Key Laboratory of Pulp and Paper Open Fund Project (201809)
  • 摘要: 本文通过使用高碘酸钠将透明质酸(HA)上的羟基氧化为醛基,合成醛基化透明质酸(OHA)。以OHA和羟丙基壳聚糖(HPCS)为原料,通过OHA上的醛基与HPCS的氨基发生席夫碱反应生成动态亚胺键,制备了OHA/HPCS自愈合水凝胶。本文通过使用FTIR、UV-vis、SEM、流变和1H NMR对OHA/HPCS自愈合水凝胶的微观形貌与性能进行表征与探究。OHA/HPCS水凝胶具有多孔结构,孔径范围在70~200 μm之间。随着OHA用量的增加,OHA/HPCS水凝胶内部的孔隙增多,孔径变小,OHA/HPCS的溶胀比逐渐变小,且降解速率变缓。在室温且无外界刺激的条件下,OHA/HPCS水凝胶在4 h内能够实现自愈合。OHA/HPCS水凝胶能够缓慢释放抗癌药物吉西他滨,药物累计释放率为70%~84%,释放时间为12天。OHA/HPCS水凝胶具有缓慢释放吉西他滨的性能,表明OHA/HPCS水凝胶在药物释放领域具有潜在的应用前景。

     

  • 图  1  OHA/HPCS自愈合水凝胶合成路线示意图

    Figure  1.  Schematic diagram of OHA/HPCS self-healing hydrogels synthesis route

    图  2  透明质酸(HA)和OHA的红外图谱

    Figure  2.  FTIR spectra of hyaluronic acid (HA) and OHA

    图  3  HPCS、HA、OHA和OHA/HPCS水凝胶的红外图谱

    Figure  3.  FTIR spectra of HPCS, HA, OHA and OHA/HPCS hydrogels

    图  4  OHA和HA的核磁共振氢谱图

    Figure  4.  1H NMR spectra of OHA and HA

    图  5  OHA1/HPCS (a)、OHA2/HPCS (b)和OHA3/HPCS (c)水凝胶的SEM图像

    Figure  5.  SEM images of OHA1/HPCS (a), OHA2/HPCS (b) and OHA3/HPCS (c) hydrogels

    图  6  OHA/HPCS水凝胶自愈合图示

    Figure  6.  Self-healing diagram of OHA/HPCS hydrogels

    图  7  OHA/HPCS 水凝胶的黏弹性能:储能模量G'和损耗模量G''随应变变化曲线

    Figure  7.  Viscoelastic properties of OHA/HPCS hydrogels: Storage modulus G' and loss modulus G" curves with strain

    图  8  OHA/HPCS自愈合水凝胶的溶胀性能曲线

    Figure  8.  Swelling performance curves of OHA/HPCS self-healing hydrogels

    图  9  OHA/HPCS水凝胶的体外降解

    Figure  9.  In vitro degradation of OHA/HPCS hydrogels

    图  10  OHA/HPCS自愈合水凝胶的药物释放曲线

    Figure  10.  Drug release curves of OHA/HPCS self-healing hydrogels

    表  1  醛基化透明质酸(OHA)/羟丙基壳聚糖(HPCS)水凝胶的实验配比

    Table  1.   Experimental ratio of oxidized hyaluronic acid (OHA)/hydroxypropyl chitosan (HPCS) hydrogel

    SampleOHA/gHPCS/gPBS/mL
    OHA1/HPCS0.030.071
    OHA2/HPCS0.050.071
    OHA3/HPCS0.070.071
    Note: PBS—Phosphate buffered saline.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-10-31
  • 修回日期:  2023-01-17
  • 录用日期:  2023-01-18
  • 网络出版日期:  2023-02-08
  • 刊出日期:  2023-10-15

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