Abstract: Natural oxidases have disadvantages such as high-cost, poor stability, and difficulty in storage. Therefore, the development of low-cost, stable and high-performance glucose biomimetic enzymes has broad application prospects. Cu-based biomimetic enzymes have received widespread attention due to their advantages of environmental friendliness, high sensitivity, and air stability. In this paper, reduced graphene oxide/Cu₄O₃ (rGO/Cu₄O₃) is fabricated using a reducing agent of corn stover extract. In non-enzyme sensors, rGO/Cu₄O₃ modified glassy carbon electrodes exhibit high sensitivity to glucose (5.6 μA·L·mmol⁻¹·cm⁻² in 0.005-1 mmol·L⁻¹, 3.1 μA·L·mmol⁻¹·cm⁻² in 1-8 mmol·L⁻¹), a wide linear range (0.005-8 mmol·L⁻¹), and high stability. The possible reason for the high glucose electrocatalytic performance is that the active Cu₄O₃ dots are distributed on the surface of rGO, exhibiting more catalytic active sites for glucose. Meanwhile, the rGO core can enhance the conductivity of the electrode, resulting in increased catalytic kinetics during Faraday processes. In addition, the antioxidants could inhibit the aggregation of the active Cu₄O₃ dots during the electrocatalytic process. This work can provide a reference for the design of glucose sensors and a possible direction for the value-added utilization of corn stover.