Abstract:
Coaxial electrospinning technology is based on the improvement of traditional electrospinning technology. The fiber material prepared by it not only has a high specific surface area, but also has a special core-shell structure, which can encapsulate active molecules, maintain their biological activity, and achieve the goal of sustained release. This article uses coaxial electrospinning technology to prepare gelatin (GEL)@poly(L-lactic acid) (PLLA) nanofiber membranes with core-shell structure. The morphology, structure, and properties of nanofiber membranes were characterized using scanning electron microscopy (SEM), laser confocal microscopy (LSCM), tensile testing, and contact angle testing. The influence of electrospinning process parameters on the morphology of nanofibers was explored, and the biocompatibility of nanofiber membranes was investigated. The results showed that the surface of the prepared GEL@PLLA core-shell nanofibers was smooth and had a clear core-shell structure. Increasing the flow velocity ratio between the core-shell layers increases the average diameter of the nanofibers from 231.41 nm to 279.49 nm. Increasing the spinning voltage gradually reduces the fiber diameter. By increasing the receiving distance, the fiber diameter decreases first and then increases. The contact angle of the GEL@PLLA fiber membrane is 126.7°, which exhibits hydrophobicity compared to pure GEL fiber membrane. The mechanical test results show that GEL@PLLA core-shell nanofibers have good flexibility and elasticity. The results of in vitro cell culture showed that bone marrow mesenchymal stem cells (BMSCs) can adhere and proliferate on the GEL@PLLA fiber membrane, indicate that the core-shell nanofiber membrane has good biocompatibility. This study can lay the foundation for the further application of fiber membranes in drug-controlled release systems and biomedical fields.