Abstract: This experimental study conducted a three-point loading test of concrete beams reinforced with a new type closed winding glass fiber-reinforced polymer (GFRP) stirrups, the effects of the form of stirrups, longitudinal reinforcement ratio, shear-span ratio and stirrups spacing on the shear behavior of concrete beams reinforced with new type closed winding GFRP stirrups were investigated. The test results indicate that the ratio of bend strength over tensile strength at the straight portion of new type closed winding GFRP stirrups is 0.81, which is 2.07 times higher than that of pultruded stirrups. When the shear-span ratio and the stirrups spacing are identical, beams with new type closed winding GFRP stirrups show improved shear performance compared with beams with pultruded stirrups. The increase in longitudinal reinforcement ratio has a minor effect on the shear capacity but could significantly improve the ductility of beams. When the spacing of the stirrups is 75 mm, new type closed winding GFRP stirrups produce greater stirrups strain and strongly confine the shear-compression zone of the concrete beam, which significantly enhances the shear capacity. The calculated shear capacities according to five fiber reinforced resin composite (FRP) reinforced concrete design codes of Chinese code (GB 50608—2020), American code (ACI 440.1R-15), Canadian code (CSA S806-12), British code (BISE—1999) and Japanese code (JSCE—1997) are significantly lower than the experimental results. It is suggested that the strain limit in design codes should be appropriately increased.