Preparation and photothermal properties of gadolinium chelated tungsten oxide nanocomposite
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摘要: 采用溶剂热法，以氯化钨(WCl6)为原料制备氧化钨(W18O49)纳米粒子，进一步利用微乳液法合成钆(Gd)螯合氧化钨(W18O49-DTPA/Gd)纳米复合材料。通过XRD、SEM、TEM对其结构和形貌进行表征，结果表明样品的结晶度高，分散性好，梭形形貌，长约为640 nm，宽约为160 nm左右，W和Gd元素均匀的分布于样品中。对W18O49-DTPA/Gd的光热性能进行测试，采用808 nm近红外光照射10 min时，样品的水溶液温度可升高到46.9℃，满足光热治疗癌症的温度要求，而对照组纯水的温度几乎无变化。样品同时具有电子计算机断层扫描(CT)成像功能，成像效果随着浓度增加而加强，与纯W18O49相比，成像效果无明显变化。与人正常干细胞(LO2细胞)共培养24 h，在不同的样品浓度下细胞存活率仍在85%以上。Abstract: W18O49 nanoparticles were first prepared by solvothermal method using tungsten chloride (WCl6) and gadolinium (Gd) nitrate (Gd(NO3)3·6H2O) as raw materials, then the gadolinium chelated tungsten oxide (W18O49-DTPA/Gd) nanocomposites were fabricated by microemulsion method. The structure and morphology were characterized by XRD, SEM and TEM. The results reveal that samples possess high crystallinity, good dispersion, spindle morphology the length is about 640 nm and the width is about 160 nm. And the W and Gd elements are distributed in the samples uniformly. The test of photothermal properties show that the temperature of the aqueous solution can increase to 46.9 C under the near-infrared light of 808 nm for 10 min that can meet the requirement of photothermal treatment of cancer. At the same time, the temperature of the pure water in the control group hardly changes. In addition, the samples also have computer tomography (CT) imaging function, the imaging effect is strengthened with the increase of the concentration. Compared with pure W18O49, the imaging effect has no obvious change. After co-cultured with samples for 24 h, The Human normal stem cells (LO2) survival rate is still above 85% at different sample concentrations.
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Key words:
- tungsten oxide /
- solvothermal method /
- microemulsion method /
- CT image /
- photothermal property
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图 1 氧化钨(W18O49)-二乙烯三胺五乙酸(DTPA)-钆(Gd)的XRD图谱和W18O49的标准PDF卡
Figure 1. XRD patterns of tungstic oxide (W18O49)-diethylenetriamine pentaacetic acid (DTPA)-gadolinium (Gd) and the standard PDF cards for W18O49
图 2 W18O49-DTPA/Gd的SEM图像和EDS能谱图: (a, b) W18O49-DTPA/Gd；(c) W元素分布；(d) Gd元素分布
Figure 2. SEM images and EDS spectrum of W18O49-DTPA/Gd: (a, b) W18O49-DTPA/Gd; (c) W element distribution; (d) Gd element distribution
图 4 W18O49-DTPA/Gd和W18O49在NIR-808 nm下的光热性能: (a)纯水；(b)浓度为0.1 mg/mL的W18O49-DTPA/Gd水溶液；(c) 浓度为1 mg/mL的W18O49-DTPA/Gd 水溶液；(d) 浓度1 mg/mL的W18O49水溶液
Figure 4. Photothermal performance of W18O49-DTPA/Gd and W18O49 at NIR-808 nm：(a) Pure water；(b) W18O49-DTPA/Gd aqueous solution with a concentration of 0.1 mg/mL；(c) W18O49-DTPA/Gd aqueous solution with a concentration of 1 mg/mL；(d) W18O49 aqueous solution with a concentration of 1 mg/mL
图 5 W18O49和W18O49-DTPA/Gd的CT对比图像
Figure 5. CT contrast images of W18O49-DTPA/Gd and W18O49
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