Preparation and biocompatibility of EDC-NHS crosslinked chitosan-polyethyleneoxide-silk fibroin electrospun nanofibers

SUN Fanchen; GUO Jing; YANG Qiang; YU Yue; ZHAO Yabo

doi:10.13801/j.cnki.fhclxb.20200421.002

Volume 37 Issue 11

Nov. 2020

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SUN Fanchen, GUO Jing, YANG Qiang, et al. Preparation and biocompatibility of EDC-NHS crosslinked chitosan-polyethyleneoxide-silk fibroin electrospun nanofibers[J]. Acta Materiae Compositae Sinica, 2020, 37(11): 2889-2896. doi: 10.13801/j.cnki.fhclxb.20200421.002

Citation:

SUN Fanchen, GUO Jing, YANG Qiang, et al. Preparation and biocompatibility of EDC-NHS crosslinked chitosan-polyethyleneoxide-silk fibroin electrospun nanofibers[J]. Acta Materiae Compositae Sinica, 2020, 37(11): 2889-2896. doi: 10.13801/j.cnki.fhclxb.20200421.002

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Preparation and biocompatibility of EDC-NHS crosslinked chitosan-polyethyleneoxide-silk fibroin electrospun nanofibers

doi: 10.13801/j.cnki.fhclxb.20200421.002

SUN Fanchen^1
,,
GUO Jing^{1, 2
,
,},
YANG Qiang^2
,,
YU Yue^{1, 2
,},
ZHAO Yabo^1
,

1.
School of Textile and Material Engineering, Dalian Polytechnic University, Dalian 116034, China
2.
Functional Fiber and its Composite Materials Engineering Technology Research Center of Liaoning Province, Dalian 116034, China

Received Date: 2019-12-04
Accepted Date: 2020-02-02

Available Online: 2020-04-21

Publish Date: 2020-11-15

Abstract

Abstract

In order to improve the spinnability of chitosan (CS)-silk fibroin (SF) composite nanofibers and solve the problem of poor dimensional stability of nanofiber membrane caused by its easy swelling, polyethylene oxide (PEO) was used to improve the spinnability of the composite fibers in this study. Electrospinning was used to prepare CS-PEO-SF nanofibers, which were cross-linked and by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). The intermolecular interaction, crystallization properties, mechanical properties and morphology of CS-PEO-SF nanofibers were characterized by FTIR, XRD, single fiber strength machine and SEM. The swelling degree and biocompatibility of CS-PEO-SF nanofibers was also characterized by cytotoxicity and cell culture tests. The results show that the addition of PEO can effectively improve the spinnability of CS-SF. The average diameter of CS-PEO-SF nanofibers is from 240 nm to 510 nm. The fiber morphology of each component is good. With the increase of SF content, the crystalline properties, tensile strength and breaking elongation of CS-PEO-SF nanofibers gradually increase. After crosslinking, the crystallinity and swelling degree of the CS-PEO-SF nanofibers decrease, and the mechanical properties increase. Before and after cross-linking, the CS-PEO-SF nanofibers has no toxicity and has good cell compatibility.
- chitosan,
- polyethylene oxide,
- silk fibroin,
- nanofibers,
- cross-linking,
- biocompatibility
Long Abstrsct
Innovation Points

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References(23)

References

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